Open AccessATP, cyclic AMP, and magnesium increase the affinity of rat striatal tyrosine hydroxylase for its cofactor.
Author(s) -
Walter Lovenberg,
E A Bruckwick,
Ingeborg Hanbauer
Publication year - 1975
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.8.2955
Subject(s) - tyrosine hydroxylase , tyrosine 3 monooxygenase , tyrosine , cofactor , oxidoreductase , phosphorylation , chemistry , biochemistry , enzyme , medicine , monooxygenase , endocrinology , biology , cytochrome p450
Treatment of rat striatal tyrosine hydroxylase [tyrosine 3-monooxygenase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating); EC 1.14.16.2] with conditions optimal for protein phosphorylation results in the reduction of the tyrosine hydroxylase Km for the cofactor 6-methyltetrahydropterin from 0.50 mM to 0.16 mM. This reaction is dependent upon ATP, 3':5'-cAMP, and Mg++ and causes a marked decrease in the sensitivity to end-product inhibition. Other brain regions and the adrenal gland show a similar response.
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