Open AccessSynthesis of RNA containing a methylated blocked 5′ terminus by HeLa nuclear homogenates
Author(s) -
Yoram Groner,
Jerard Hurwitz
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.8.2930
Subject(s) - hela , rna , in vitro , chemistry , microbiology and biotechnology , stereochemistry , biochemistry , biology , gene
RNA synthesizedin vitro by HeLa nuclei contains a variety of blocked methylated 5′-terminal structures that can be grouped into four general types: (a ) m7 GppNm p...; (b ) m7 GppNm pMm p...; (c ) m7 GpppNm p...; and (d ) m7 GpppNm pMm .... Both Nm and Mm can be either one of the four 2′-O -methylated nucleosides. The relative amounts of the Nm species are G = 45%, A = 25%, U = 20%, and C = 10%.