Open AccessAn octamer of histones in chromatin and free in solution.
Author(s) -
Jean O. Thomas,
Roger D. Kornberg
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.7.2626
Subject(s) - histone octamer , histone , chromatin , chemistry , dissociation (chemistry) , ionic strength , biophysics , nucleosome , biochemistry , crystallography , biology , dna , aqueous solution
Crosslinking with dimethyl suberimidate reveals a chain of histone octamers in chromatin. The octamer can be isolated free in solution at high ionic strength and pH. The identification of dimers formed by crosslinking reveals two or more contacts of each histone with others within the octamer. The molecular weight (110,000) and pattern of dissociation of the octamer are compatible with the composition (F2A1)2(F3)2(F2A2)2(F2B)2.