Open AccessTemperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation.
Author(s) -
Joel B. Kirschbaum,
Ivan V. Claeys,
Sergio Nasi,
Bruce Molholt,
Jeffrey H Miller
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.6.2375
Subject(s) - mutant , microbiology and biotechnology , beta (programming language) , protein subunit , biology , rna polymerase , polymerase , rna , gene , specificity factor , biochemistry , computer science , programming language
A temperature-sensitive mutant having a lethal mutation in the gene for the beta subunit of RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) exhibits an apparent 2- to 3-fold decrease in the rates of both beta and beta' subunit synthesis at the non-permissive temperature, relative to total protein. In contrast, a temperature-sensitive mutant with a lethal mutation in the gene encoding beta' has a 5- to 6-fold increase in the rates of beta and beta' synthesis at 42 degrees. These beta and beta' mutants also exhibit rapid degradation of both subunits at the high temperature.