Open AccessDetermination of genes, restriction sites, and DNA sequences surrounding the 6S RNA template of bacteriophage lambda.
Author(s) -
Jeffrey Sklar,
P. Yot,
Sherman M. Weissman
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.5.1817
Subject(s) - biology , rna , genetics , restriction enzyme , transcription (linguistics) , microbiology and biotechnology , rna polymerase , dna , bacteriophage , nucleic acid sequence , gene , escherichia coli , linguistics , philosophy
A major product of the transcription of bacteriophage lambda DNA in vitro is the 6S RNA. This article presents a detailed mapping of restriction endonuclease cleavage sites about the region of the 6S RNA template within the lambda genome. Restriction fragments defined by these sites have been used to localize the 6S RNA template within the physical and genetic maps of the lambda genome. Nucleotide sequence analysis of one of these fragments has largely confimed the nucleotide sequence of the 6S RNA reported previously and has indicated the sequence of DNA that immediately follows the 6S RNA template. This article reports the nucleotide sequence following a known site of transcription termination by RNA polymerase of Escherichia coli.