Open AccessVisualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum.
Author(s) -
M Clarke,
Gerald Schatten,
Daniel Mazia,
James A. Spudich
Publication year - 1975
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.5.1758
Subject(s) - dictyostelium discoideum , mycetozoa , microbiology and biotechnology , actin , cytoplasm , biophysics , dictyostelium , myosin , biology , membrane , chemistry , biochemistry , gene
Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin.
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