Open AccessRNA synthesis in isolated brian nuclei after administration of d-lysergic acid diethylamide (LSD) in vivo.
Author(s) -
Ian R. Brown
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.3.837
Subject(s) - lysergic acid diethylamide , rna , in vivo , transcription (linguistics) , biology , microbiology and biotechnology , biochemistry , chemistry , pharmacology , gene , genetics , receptor , linguistics , philosophy , serotonin
RNA synthesis in isolated brain nuclei was analyzed 2.5 hr after the intravenous administration of d-lysergic acid diethylamide (LSD) to young rabbits. The drug stimulated transcription by 54% in brain stem nuclei and by 13% in cerebral hemisphere nuclei expressed over saline controls. Both nucleoplasmic and nucleolar RNA synthesis were increased. The main activity in the isolated nuclei assay was due to nucleoplasmic RNA polymerase, since alpha-amanitin reduced synthesis by over 70% in either drug or control treatments.