Open AccessReplication of colicin E1 plasmid DNA added to cell extracts.
Author(s) -
Jun-ichi Tomizawa,
Yasumasa Sakakibara,
Tsuyoshi Kakefuda
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.3.1050
Subject(s) - semiconservative replication , plasmid , colicin , dna replication , origin of replication , biology , cole1 , escherichia coli , plasmid preparation , dna , dna synthesis , eukaryotic dna replication , rolling circle replication , microbiology and biotechnology , genetics , gene , pbr322
Closed-circular DNA of colicin E1 plasmid can undergo a round of semiconservative replication when added to an extract of Escherichia coli. Extracts of cells that do not carry the plasmid are able to perform complete replication of the plasmid. Replication requires de novo RNA synthesis but not protein synthesis.