Replication of colicin E1 plasmid DNA added to cell extracts. | Zendy

Jun-ichi Tomizawa | Zendy; Yoshimasa Sakakibara | Zendy; T Kakefuda | Zendy

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Replication of colicin E1 plasmid DNA added to cell extracts.

Author(s) -

Jun-ichi Tomizawa,

Yoshimasa Sakakibara,

T Kakefuda

Publication year - 1975

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.72.3.1050

Subject(s) - semiconservative replication , plasmid , colicin , dna replication , origin of replication , biology , cole1 , escherichia coli , plasmid preparation , dna , dna synthesis , eukaryotic dna replication , rolling circle replication , microbiology and biotechnology , genetics , gene , pbr322

Closed-circular DNA of colicin E1 plasmid can undergo a round of semiconservative replication when added to an extract of Escherichia coli. Extracts of cells that do not carry the plasmid are able to perform complete replication of the plasmid. Replication requires de novo RNA synthesis but not protein synthesis.

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