Hamster alpha-amanitine-resistant RNA polymerase II able to transcribe polyoma virus genome in somatic cell hybrids. | Zendy

Paolo Amati | Zendy; Francesco Blasi | Zendy; Umberto di Porzio | Zendy; Anna Riccio | Zendy; Cinzia Traboni | Zendy

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Hamster alpha-amanitine-resistant RNA polymerase II able to transcribe polyoma virus genome in somatic cell hybrids.

Author(s) -

Paolo Amati,

Francesco Blasi,

Umberto di Porzio,

Anna Riccio,

Cinzia Traboni

Publication year - 1975

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.72.2.753

Subject(s) - biology , microbiology and biotechnology , baby hamster kidney cell , hamster , rna , transcription (linguistics) , somatic cell , polymerase , virology , rna polymerase ii , mutant , rna polymerase , rna dependent rna polymerase , cell culture , virus , dna , genetics , gene , gene expression , promoter , linguistics , philosophy

A hamster cell line resistant to alpha-amanitine has been isolated (alpha-am-r, BHK-T6-G-1). Cell extracts of this mutant have an alpha-amanitine-resistant RNA polymerase II (nucleosidetriphosphate: RNA nucleotidyl-transferase, EC 2.7.7.6) activity as shown by DEAE-cellulose column chromatography. This mutation is dominant in interspecific hybrids with 3T3 mouse cells. In such hybrids polyoma virus can grow with equal efficiency in the presence or absence of the drug, thus indicating that the RNA polymerase of the unsusceptible parental cell can participate in the correct transcription of the viral genome.

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