The in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product. | Zendy

Charles Gilvarg | Zendy; F.J. Bollum | Zendy; C. Weissmann | Zendy

Open Access

The in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product.

Author(s) -

Charles Gilvarg,

F.J. Bollum,

C. Weissmann

Publication year - 1975

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.72.2.428

Subject(s) - rna , in vitro , biology , infectivity , microbiology and biotechnology , virology , spheroplast , chemistry , biochemistry , virus , escherichia coli , gene

Terminal riboadenylate transferase, purified from calf thymus, has been used to add a poly(A) extension to the 3' end of Qbeta RNA. The modified Qbeta RNA retains full infectivity in a spheroplast assay system. However, the progeny viruses do not contain poly(A) termini, indicating an in vivo rectification of the in vitro alteration.

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