Open AccessThe in vitro addition of a polyadenylate sequence to the 3' end of phage Qbeta RNA and the biological activity of the product.
Author(s) -
Charles Gilvarg,
F. J. Bollum,
C. Weissmann
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.2.428
Subject(s) - rna , in vitro , biology , infectivity , microbiology and biotechnology , virology , spheroplast , chemistry , biochemistry , virus , escherichia coli , gene
Terminal riboadenylate transferase, purified from calf thymus, has been used to add a poly(A) extension to the 3' end of Qbeta RNA. The modified Qbeta RNA retains full infectivity in a spheroplast assay system. However, the progeny viruses do not contain poly(A) termini, indicating an in vivo rectification of the in vitro alteration.