Open AccessLambda repressor turns off transcription of its own gene.
Author(s) -
Barbara J Meyer,
Dennis G. Kleid,
Mark Ptashne
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.12.4785
Subject(s) - repressor , transcription (linguistics) , gene , biology , mutant , general transcription factor , microbiology and biotechnology , yy1 , promoter , genetics , transcription factor , gene expression , linguistics , philosophy
We report transcription in vitro of the lambda repressor gene, cI, using specific restriction endonuclease fragments as templates. This transcription is repressed by lambda repressor. Moreover, we report the sequence change caused by a cI promoter mutation. This change is located between two repressor binding sites in the rightward operator (OR). Transcription studies using mutant templates indicate that repressor bound to two sites in OR regulates transcription of gene tof, and repressor bound to the remaining site(s) controls transcription of cI.