Open AccessTranslation of messenger ribonucleic acid from isolated pancreatic islets and human insulinomas.
Author(s) -
Cecil C. Yip,
ChoyLeong Hew,
H W Hsu
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.12.4777
Subject(s) - proinsulin , rna , xenopus , pancreatic islets , gel electrophoresis , sodium dodecyl sulfate , microbiology and biotechnology , messenger rna , biology , biochemistry , immunoprecipitation , leucine , insulin , islet , chemistry , medicine , endocrinology , amino acid , gene
RNA preparations from isolated rat pancreatic islets and from human insulinomas were injected into oocytes of Xenopus laevis which were then incubated with [3H]leucine. Acid-ethanol extracts of the oocytes were immunoprecipitated with anti-insulin serum using the double antibody technique. Sodium dodecyl sulfate disc gel electrophoresis of the immunoprecipitates showed the presence of an insulin-displaceable immunoreactive material with a molecular weight of about 18,000 in extracts from oocytes injected with RNA of 9-11 S. This immunoreactive product was not detected in extracts from oocytes injected with buffer or 4-8S RNA or RNA heavier than 11 S. These observations suggest the involvement of a precursor larger than proinsulin in the biosynthesis of insulin.