Open AccessAdenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA.
Author(s) -
Steven L. Bachenheimer,
James Darnell
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.11.4445
Subject(s) - rna , ecori , dna , transcription (linguistics) , microbiology and biotechnology , biology , small nuclear rna , messenger rna , restriction enzyme , base pair , post transcriptional modification , rna editing , non coding rna , genetics , gene , linguistics , philosophy
The order of transcription and the length of nascent RNA transcripts from adenovirus-2 (Ad-2) DNA in the nucleus of infected cells has been deduced by labeling the growing RNA chains in vivo for a very brief period, separating the RNA on the basis of size, and hybridizine to the ordered EcoRI restriction endonuclease fragments derived from Ad-2 DNA. The majority of the virus-specific RNA molecules are synthesized as very high-molecular-weight units beginning at a common point at least 25-30,000 base pairs from one end of the Ad-2 DNA. These molecules can be reduced in size without further RNA synthesis. The experiments indicate the obligatory origin of Ad-2 mRNA from a high-molecular-weight precursor molecule.