A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules. | Zendy

H.J. Vollenweider | Zendy; José M. Sogo | Zendy; T. Köller | Zendy

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A routine method for protein-free spreading of double- and single-stranded nucleic acid molecules.

Author(s) -

H.J. Vollenweider,

José M. Sogo,

T. Köller

Publication year - 1975

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.72.1.83

Subject(s) - nucleic acid , dna , rna , reagent , chemistry , biochemistry , biophysics , monolayer , polymerase , bacteriophage , biology , organic chemistry , escherichia coli , gene

A protein-free nucleic acid preparation method for electron microscopy is described. The basic procedure is very similar to the classical protein monolayer spreading techniques. The carrier protein (usually cytochrome c) is replaced by benzyldimethylalkylammonium chloride. Both the hypophase method and the microdiffusion or droplet method can be applied with this compound. Unlike cytochrome c, benzyldimethylalkylammonium chloride does not lead to any apparent thickening of the nucleic acid strands. Partially denatured DNA spread with this reagent shows a loosened structure with a foamy appearance in the regions previously considered to be "unmelted," which open up locally into melted loops of different size. Specifically bound proteins, such as RNA polymerase on bacteriophage T7 DNA, can be detected unambiguously.

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