Open AccessCollagenase Production by Endotoxin-Activated Macrophages
Author(s) -
Larry M. Wahl,
Sharon M. Wahl,
Stephan E. Mergenhagen,
George R. Martin
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.9.3598
Subject(s) - collagenase , lipopolysaccharide , cycloheximide , macrophage , lipid a , exudate , microbiology and biotechnology , chemistry , biochemistry , enzyme , biology , in vitro , protein biosynthesis , immunology , botany
Peritoneal exudate macrophages, when exposed to bacterial lipopolysaccharide in culture, were found to produce collagenase (EC 3.4.24.3). This enzyme was not detected in extracts of the macrophages or in media from nonstimulated macrophage cultures. Lipidcontaining fractions of the lipopolysaccharide, including a glycolipid from the rough mutant ofSalmonella minnesota (R595) and lipid A, were potent stimulators of collagenase production. The lipid-free polysaccharide fraction had no effect. Cycloheximide prevented the production of collagenase by endotoxin-treated macrophages, suggesting that it was newly synthesized.