Open AccessDifferentiation of Cell Membranes in Cultures of Embryonic Chick Breast Muscle
Author(s) -
Joav Prives,
Bruce M. Paterson
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.8.3208
Subject(s) - myogenesis , myocyte , microbiology and biotechnology , acetylcholinesterase , biology , cellular differentiation , cytoplasm , membrane , acetylcholine , pyrophosphate , cell fusion , cyclase , cell , biochemistry , receptor , endocrinology , enzyme , gene
Three components of differentiated muscle membrane, the acetylcholine receptor, acetylcholinesterase (EC 3.1.1.7; acetylcholine hydrolase), and adenylate cyclase [EC 4.6.1.1; ATP pyrophosphate-lyase (cyclizing)], appear simultaneously during myogenesis in cultures of embryonic chick muscle, after the main period of rapid cell fusion. However, unlike the cytoplasmic proteins of differentiated muscle, the elaboration of these membrane components is unaltered when fusion is blocked by lowering the calcium concentration in the medium. These results suggest that membrane differentiation and cytoplasmic differentiation are regulated independently during muscle development.