Open AccessLong Pyrimidine Tracts of L-Cell DNA: Localization to Repeated DNA
Author(s) -
Neil A. Straus,
H.C. Birnboim
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.8.2992
Subject(s) - dna , thymidine , biology , hela , microbiology and biotechnology , pyrimidine , cell , biochemistry
Like HeLa DNA, L-cell DNA contains a significant number of unexpectedly long pyrimidine tracts. 1.3% of the thymidine residues of L-cell DNA are found in these long polypyrimidine tracts. Analysis of L-cell DNA fractions with different rates of reassociation indicates that polypyrimidine tracts are associated with “repeated” DNA. Longer pyrimidine tracts appear to have a higher repetition frequency than shorter tracts, and some may be repeated as many as 5 × 104 times in L-cell DNA.