Open AccessControl of the Initiation of DNA Synthesis in 3T3 Cells: Low-Molecular-Weight Nutrients
Author(s) -
Robert W. Holley,
Josephine A. Kiernan
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.8.2942
Subject(s) - dna synthesis , 3t3 cells , dna , cell culture , biochemistry , limiting , phosphate , cell cycle , nutrient , cell , biology , microbiology and biotechnology , chemistry , transfection , genetics , mechanical engineering , ecology , engineering
Sparse 3T3 cells, in excess serum, become quiescent in the early G1 (or G0 ) phase of the cell cycle if the cells are cultured in low concentrations of amino acids, glucose, or phosphate ion. These quiescent cells then initiate DNA synthesis if the concentration of the limiting nutrient is increased. In normal medium, DNA synthesis in the same 3T3 cell line is controlled by serum factors. The results demonstrate the complexity of the control by external agents of DNA synthesis in these “normal,” density-dependent cells.