Open AccessIsolation and Some Properties of the Genes Coding for Histone Proteins
Author(s) -
Max L. Birnstiel,
John L. Telford,
Eric S. Weinberg,
Darrel W. Stafford
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.7.2900
Subject(s) - histone , dna , biology , gene , sea urchin , dna sequencing , genetics , biochemistry , computational biology , microbiology and biotechnology
The DNA coding for histone proteins, together with the intervening spacer DNA, has been isolated from the sea urchin,Psammechinus miliaris , by repeated centrifugation on CsCl-asctinomycin gradients. The biophysical data show that a major portion of the spacer is 37% G+C and very divergent in sequence, while the DNA segments coding for histone proteins are high in G+C and have diverged to a lesser extent within the species.