Cell-free Synthesis of Adenovirus 2 Proteins Programmed by Fractionated Messenger RNA: A Comparison of Polypeptide Products and Messenger RNA Lengths | Zendy

Carl W. Anderson | Zendy; James B. Lewis | Zendy; John F. Atkins | Zendy; R.F. Gesteland | Zendy

Open Access

Cell-free Synthesis of Adenovirus 2 Proteins Programmed by Fractionated Messenger RNA: A Comparison of Polypeptide Products and Messenger RNA Lengths

Author(s) -

Carl W. Anderson,

James B. Lewis,

John F. Atkins,

R.F. Gesteland

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.7.2756

Subject(s) - rna , messenger rna , protein biosynthesis , microbiology and biotechnology , biology , translation (biology) , polyacrylamide gel electrophoresis , gel electrophoresis , cell free protein synthesis , ribosome , cell free system , cell culture , five prime cap , biochemistry , chemistry , non coding rna , gene , enzyme , genetics

Cytoplasmic RNA extracted from human tissue culture cells infected with adenovirus type 2 was used to program protein synthesis in a cell-free system derived from mammalian cells. Analysis of the protein product by polyacrylamide gel electrophoresis revealed ten adenovirus-specific polypeptides. Five of these were further identified by analysis of tryptic peptides. Translation of RNA fractionated by sedimentation through sucrose gradients containing formamide demonstrated seven size classes of RNA, each of which programmed the synthesis of only one or two virus-specific polypeptides. Six of the virus-specific polypeptides were translated from RNAs much larger than expected for the size of the polypeptide.

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