Open AccessHepatitis B Antigen: Antigenic Sites Related to Human Serum Proteins Revealed by Affinity Chromatography
Author(s) -
Neurath Ar,
Prince Am,
Arnold Lippin
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.7.2663
Subject(s) - antigen , affinity chromatography , antibody , chemistry , hepatitis b , biochemistry , microbiology and biotechnology , immunoglobulin g , hepatitis , enzyme , biology , virology , immunology
Hepatitis B antigen-associated particles, isolated from sera of antigen carriers, were submitted to affinity chromatography on columns of insolubilized antibodies to normal human plasma. The particles adsorbed to the immunosorbent at pH 7.2 and were subsequently eluted at pH 2.2. Exposure of the particles to 8 M urea, 5 M KI, pH 2.2, detergents, organic solvents, or proteolytic enzymes failed to prevent their subsequent adsorption to the immunosorbent. This suggests that antigenic determinants related to human plasma proteins are constituent components of hepatitis B antigen-associated particles. These determinants are distinct from the group-specific (a ) and subtype-specific (d ory ) sites of the hepatitis B antigen and appear to be related to antigenic specificities on prealbumin, albumin, apolipoproteins C and D, and the γ-chain of immunoglobulin G.