Hepatitis B Antigen: Antigenic Sites Related to Human Serum Proteins Revealed by Affinity Chromatography | Zendy

A. R. Neurath | Zendy; Alfred M. Prince | Zendy; A. Lippin | Zendy

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Hepatitis B Antigen: Antigenic Sites Related to Human Serum Proteins Revealed by Affinity Chromatography

Author(s) -

A. R. Neurath,

Alfred M. Prince,

A. Lippin

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.7.2663

Subject(s) - antigen , affinity chromatography , antibody , chemistry , hepatitis b , biochemistry , microbiology and biotechnology , immunoglobulin g , hepatitis , enzyme , biology , virology , immunology

Hepatitis B antigen-associated particles, isolated from sera of antigen carriers, were submitted to affinity chromatography on columns of insolubilized antibodies to normal human plasma. The particles adsorbed to the immunosorbent at pH 7.2 and were subsequently eluted at pH 2.2. Exposure of the particles to 8 M urea, 5 M KI, pH 2.2, detergents, organic solvents, or proteolytic enzymes failed to prevent their subsequent adsorption to the immunosorbent. This suggests that antigenic determinants related to human plasma proteins are constituent components of hepatitis B antigen-associated particles. These determinants are distinct from the group-specific (a ) and subtype-specific (d ory ) sites of the hepatitis B antigen and appear to be related to antigenic specificities on prealbumin, albumin, apolipoproteins C and D, and the γ-chain of immunoglobulin G.

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