Nuclear Ribonucleoprotein Complexes Containing Polyadenylate from Mouse Ascites Cells | Zendy

Thomas J. Quinlan | Zendy; Peter B. Billings | Zendy; Terence E. Martin | Zendy

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Nuclear Ribonucleoprotein Complexes Containing Polyadenylate from Mouse Ascites Cells

Author(s) -

Thomas J. Quinlan,

Peter B. Billings,

Terence E. Martin

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.7.2632

Subject(s) - ribonucleoprotein , rna , ribonucleoprotein particle , molecular mass , heterogeneous nuclear ribonucleoprotein , chemistry , nucleoprotein , ascites , cell nucleus , biochemistry , microbiology and biotechnology , biology , cytoplasm , enzyme , dna , gene , medicine , gastroenterology

Nuclear poly(A)-containing RNA of mouse ascites cells can be extracted in the form of 15-17S ribonucleoprotein complexes under conditions in which the bulk of the heterogeneous nuclear RNA is released as 30S complexes. The poly(A)-containing fraction of nuclear extracts has been resolved into two distinct components, 15 and 17 S; neither contains the two polypeptides of 30S ribonucleoprotein. The 17S particle contains approximately six polypeptide species of molecular masses 17,000-30,000 daltons. The 15S complex has four distinct polypeptides of higher molecular mass, including a prominent 80,000-dalton species.

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