Open AccessThe Mechanism of Viral Replication. Structure of Replication Complexes of Encephalomyocarditis Virus
Author(s) -
Sigrid S. Thach,
D C Dobbertin,
Charles B. Lawrence,
Fred Golini,
Robert E. Thach
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.6.2549
Subject(s) - rna , virus , viral replication , protein filament , replication (statistics) , biology , virology , double stranded , microbiology and biotechnology , chemistry , biophysics , dna , genetics , gene
The structure of the purified replicative intermediate of encephalomyocarditis virus was determined by electron microscopy. Approximately 80% of the replicative intermediate complexes were characterized by a filament of double-stranded RNA of widely variable length, which had a “bush” of single-stranded RNA at one end. In many examples one or more additional single-stranded bushes were appended internally to the double-stranded RNA filament. These results support the view that before deproteinization, replicative intermediate contains little if any double-stranded RNA.