Activity of Empty, Headlike Particles for Packaging of DNA of Bacteriophage λ In Vitro | Zendy

Barbara Höhn | Zendy; T. Höhn | Zendy

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Activity of Empty, Headlike Particles for Packaging of DNA of Bacteriophage λ In Vitro

Author(s) -

Barbara Höhn,

T. Höhn

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.6.2372

Subject(s) - lysogenic cycle , complementation , bacteriophage , mutant , escherichia coli , dna , microbiology and biotechnology , lambda phage , in vitro , centrifugation , biology , biochemistry , gene

A precursor head of phage lambda is synthesized after induction of cells lysogenic for λD- F- and λA - (head-defective) mutants. This precursor head can be assayed by complementationin vitro and can be purified by CsCl gradient centrifugation and sucrose gradient sedimentation. The precursor head contains no DNA and has the same dimensions as the petit λ particle. It can be packed with λ DNA in an extract from inducedEscherichia coli lysogenic for a λE - mutant.

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