Open AccessIn Vitro Repair of Apurinic Sites in DNA
Author(s) -
Walter G. Verly,
Françis Gossard,
Philippe Crine
Publication year - 1974
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.6.2273
Subject(s) - ap site , dna ligase , dna (apurinic or apyrimidinic site) lyase , dna repair , microbiology and biotechnology , dna polymerase , dna , endonuclease , dna polymerase i , biology , dna polymerase ii , ap endonuclease , chemistry , biochemistry , polymerase chain reaction , gene , reverse transcriptase
Apurinic sites disappear from DNA during an incubation of the DNA with theEscherichia coli endonuclease specific for apurinic sites, DNA polymerase I (EC 2.7.7.7.), and T4 ligase (EC 6.5.1.1). Omission of any one of these three enzymes and, in particular, omission of the endonuclease specific for apurinic sites prevents thisin vitro repair.
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