Open AccessReplication and Transcription of Eukaryotic DNA in Esherichia coli
Author(s) -
John F. Morrow,
Stanley N. Cohen,
Annie Chang,
Herbert W. Boyer,
Howard M. Goodman,
Robert B. Helling
Publication year - 1974
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.5.1743
Subject(s) - biology , plasmid , recombinant dna , microbiology and biotechnology , ecori , dna , escherichia coli , in vitro recombination , restriction enzyme , transcription (linguistics) , dna replication , genetics , molecular cloning , gene , complementary dna , linguistics , philosophy
Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.
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