Open AccessSynthesis of Nerve Growth Factor by L and 3T3 Cells in Culture
Author(s) -
Joël Oger,
Barry G. W. Arnason,
Nicholas J. Pantazis,
James R. Lehrich,
Michael Young
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.4.1554
Subject(s) - nerve growth factor , neurite , 3t3 cells , tissue culture , biology , bacteriophage , growth factor , cell culture , endocrinology , medicine , microbiology and biotechnology , chemistry , in vitro , biochemistry , transfection , escherichia coli , receptor , genetics , gene
Mouse submaxillary gland nerve growth factor (NGF) has been covalently joined to bacteriophage and the resulting phage conjugates remain biologically active in stimulating neurite extension from sensory ganglia. A sensitive bacteriophage immunoassay has been developed to measure concentrations of NGF as low as 1 ng/ml. With this method, we find that mouse L and 3T3 cells in culture produce a biologically active nerve growth factor that is immunologically similar if not identical to mouse submaxillary gland NGF. Since L cells are known to be a source of “conditioned medium” for tissue culture, it could be that one or more of the conditioning factor activities secreted by these cells are due to NGF itself.