The Effect of Pressure on the Molecular Complex of Isoalloxazine and Adenine | Zendy

Gregorio Weber | Zendy; Fumio Tanaka | Zendy; Byron Y. Okamoto | Zendy; H. G. Drickamer | Zendy

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The Effect of Pressure on the Molecular Complex of Isoalloxazine and Adenine

Author(s) -

Gregorio Weber,

Fumio Tanaka,

Byron Y. Okamoto,

H. G. Drickamer

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.4.1264

Subject(s) - flavin mononucleotide , flavin adenine dinucleotide , flavin group , chemistry , fluorescence , complex formation , denaturation (fissile materials) , enzyme , cofactor , biochemistry , inorganic chemistry , nuclear chemistry , physics , quantum mechanics

The effect of pressure to 10 kilobars on the fluorescence characteristics of flavin mononucleotide (FMN), flavin-adenine dinucleotide (FAD), and on complexes of FMN with adenylic acid (AMP), and with I- has been studied. The properties measured include peak location, fluorescence yield, and lifetime. From these results the equilibrium constantK and the rate constantk + * for complex formation were evaluated as a function of pressure. The pressure dependence of these coefficients shows that the volume of the system decreases upon complex formation and that there is an expansion upon formation of the activated complex (ΔV ‡ is positive). The implications of these results for protein denaturation are mentioned.

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