Open AccessIsolation of Heterogeneous Circular DNA from Induced Lysogens of Bacteriophage Mu-1
Author(s) -
Barbara T. Waggoner,
Nélida S. González,
Anna M.W. Taylor
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.4.1255
Subject(s) - lysogen , bacteriophage , dna , microbiology and biotechnology , circular dna , molecule , biology , lysogenic cycle , phagemid , bacteriophage mu , genome , circular bacterial chromosome , genetics , chemistry , dna replication , gene , escherichia coli , organic chemistry
Covalently-closed circular DNA molecules are formed after induction of phage Mucts4 and after infection with phage Mucts4 . The circular molecules obtained after induction have a molecular length range from 36.5 to 156.7 kilobases as measured by electron microscopic techniques. These heterogeneous molecules have no consistent correlation to exact multiples of a Mu genome equivalent (37.3 ± 1.2 kilobases). Direct evidence is given that these molecules contain phage Mu DNA that is covalently linked to other DNA sequences.