Open AccessAdriamycin and Daunorubicin Inhibition of Mutant T4 DNA Polymerases
Author(s) -
Myron F. Goodman,
Maurice J. Bessman,
Nicholas R. Bachur
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.4.1193
Subject(s) - dna polymerase , daunorubicin , polymerase , ethidium bromide , microbiology and biotechnology , exonuclease , biology , mutant , dna , enzyme , biochemistry , aphidicolin , dna polymerase ii , chemistry , genetics , polymerase chain reaction , reverse transcriptase , gene , leukemia
The anticancer drugs, adriamycin and daunorubicin, as well as two other DNA reagents, ethidium bromide and 9-aminoacridine, all exert a differential inhibitory effect on nucleotide incorporation for purified DNA polymerases induced by mutant and wild-type bacteriophage T4. When compared with DNA polymerase of wild-type phage, antimutator enzymes are inhibited to a far greater extent and mutator enzymes to a lesser extent. In contrast, the polymerase-associated 3′-exonuclease activities of wild type and mutants are also inhibited by the compounds but nondifferentially.