Purification and Properties of Two RNA Polymerases from Physarum polycephalum | Zendy

Ann Burgess | Zendy; Richard R. Burgess | Zendy

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Purification and Properties of Two RNA Polymerases from Physarum polycephalum

Author(s) -

Ann Burgess,

Richard R. Burgess

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.4.1174

Subject(s) - physarum polycephalum , divalent , enzyme , sodium dodecyl sulfate , biochemistry , physarum , rna , dna , polymerase , chemistry , sodium , biology , gene , organic chemistry

Two RNA polymerases have been purified from the slime mold Physarum polycephalum, one sensitive and one resistant to alpha-amanitin. Both enzymes are more active with denatured DNA than native DNA as a template and prefer Mn(++) rather than Mg(++) as a divalent cation. The alpha-amanitin-sensitive enzyme shows maximum activity at 0.15 M KCl, whereas the resistant enzyme is most active at very low ionic strength. Analysis of the resistant enzyme on polyacrylamide gels containing sodium dodecyl sulfate shows two subunits present in a 1:1 ratio with molecular weights of 205,000 and 125,000.

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