Isolation and Analysis of Somatic Hybrids Derived from Two Human Diploid Cells | Zendy

Barbara R. Migeon | Zendy; Robert A. Norum | Zendy; Cheryl M. Corsaro | Zendy

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Isolation and Analysis of Somatic Hybrids Derived from Two Human Diploid Cells

Author(s) -

Barbara R. Migeon,

Robert A. Norum,

Cheryl M. Corsaro

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.3.937

Subject(s) - ploidy , biology , cell fusion , hypoxanthine , somatic fusion , wi 38 , polyploid , amethopterin , somatic cell , hybrid , sendai virus , thymidine , fibroblast , microbiology and biotechnology , hypoxanthine guanine phosphoribosyltransferase , genetics , cell culture , biochemistry , virus , dna , gene , enzyme , immunology , botany , methotrexate , mutant

Lesch-Nyhan fibroblasts and normal human leukocytes with different glucose-6-phosphate dehydrogenase genotypes were fused by Sendai virus. Clones were isolated on the basis of their resistance to a medium containing hypoxanthine, amethopterin, and thymidine and ability to proliferate in monolayer culture. These mononuclear cells (1 ) incorporated [3 H]hypoxanthine, (2 ) expressed the glucose-6-phosphate dehydrogenase heteropolymer, and (3 ) were polyploid. Therefore, hybrids can originate from the fusion of two diploid human cells. X chromosome inactivation did not occur in these hybrid cells of male origin. The hybrids expressed both parental genomes and exhibited the senescence and contact feeding characteristic of the human skin fibroblast.

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