Open AccessLocalization of the Stringent Protein of Escherichia coli on the 50S Ribosomal Subunit
Author(s) -
Subbanaidu Ramagopal,
Bernard D. Davis
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.3.820
Subject(s) - ribosome , 50s , 30s , ribosomal rna , ribosomal protein , protein subunit , eukaryotic ribosome , polysome , chemistry , centrifugation , escherichia coli , eukaryotic large ribosomal subunit , biochemistry , biology , rna , gene
The “stringent” protein of the ribosome, required for its synthesis of (p)ppGpp, is readily lost during zonal centrifugation. However, enough can be retained to permit its qualitative localization. It is found in native 50S subunits, runoff 70S ribosomes, and polysomes, but not in native 30S subunits. This protein, therefore, appears to be attached to the 50S moiety of the ribosome, and it may be a constant (though easily removed) component of that structure rather than a factor that joins and leaves during the ribosome cycle.