The Secretion of α-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro | Zendy

David S. King | Zendy; Walter E. Bollenbacher | Zendy; David W. Borst | Zendy; Wayne V. Vedeckis | Zendy; John D. O’Connor | Zendy; P. I. Ittycheriah | Zendy; L Gilbert | Zendy

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The Secretion of α-Ecdysone by the Prothoracic Glands of Manduca sexta In Vitro

Author(s) -

David S. King,

Walter E. Bollenbacher,

David W. Borst,

Wayne V. Vedeckis,

John D. O’Connor,

P. I. Ittycheriah,

L Gilbert

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.3.793

Subject(s) - manduca sexta , prothoracic gland , manduca , ecdysone , sphingidae , secretion , biology , endocrinology , in vitro , medicine , insect , hormone , biochemistry , botany

Prothoracic glands of tobacco hornworm larvae culturedin vitro secrete into the culture medium a substance which was active in ecdysone bioassays and determined to be ecdysone-like by radioimmunoassay. The prothoracic glands appear to be the sole source of this substance. The material was identified as α-ecdysone by thin-layer, gas-liquid, and high-resolution liquid chromatography and mass spectrometry. No other active moulting principles were present in the medium. It appears that the prothoracic gland secretes a prohormone, α-ecdysone, which is subsequently converted into the active moulting hormone, β-ecdysone, in other insect tissues.

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