Open AccessIntroduction of Specific Cleavages into RNAs of RNA Bacteriophages for Determination of Base Sequences
Author(s) -
Motohiro Fuke
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.3.742
Subject(s) - rna , ribonuclease , ribonuclease t1 , small nucleolar rna , non coding rna , biology , base pair , ribonuclease iii , computational biology , chemistry , genetics , microbiology and biotechnology , dna , rnase p , gene , rna interference
Large fragments of R17, MS2, and Qβ RNAs were obtained reproducibly by limited digestion by ribonuclease T1. After digestion of R17 and MS2 RNAs, two pairs of large fragments were obtained that probably resulted from specific cleavages of the whole molecules. The cleavages of MS2 RNA were produced at points 36 and 47% from the 5′ end.