Open AccessMembrane Molecules Determined by the H-2 Associated Immune Response Region: Isolation and Some Properties
Author(s) -
Susan E. Cullen,
Chella S. David,
D C Shreffler,
Stanley G. Nathenson
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.3.648
Subject(s) - antiserum , microbiology and biotechnology , antigen , dimer , glycoprotein , sodium dodecyl sulfate , gel electrophoresis , polyacrylamide gel electrophoresis , immune system , chemistry , biochemistry , antibody , molecular mass , biology , enzyme , immunology , organic chemistry
Membrane associated molecules that are probably glycoproteins could be specifically precipitated from NP-40 detergent solubilized extracts of radiolabeled mouse spleen or lymph node cells by antisera produced in congenic strain combinations differing only in theIr gene region which is linked to theH-2 genes. TheseIr region products were designated Lna (lymph node antigen) to conform to previous serological work.Sodium dodecyl sulfate—polyacrylamide gel electrophoresis of unreduced specific immune precipitates revealed the presence of a possible dimer form, while reduced samples showed only a single peak equivalent to 30,000 daltons. Thus the Lna molecules are clearly distinct from theH-2D andH-2K molecules, which are about 45,000 daltons. Anti-Lna antibodies of different specificity can be present in a single serum; there were at least two separate antigen molecules present in one haplotype tested.