Effect of 5-Bromodeoxyuridine on Chromatin Transcription in Confluent Fibroblasts | Zendy

Bridget T. Hill | Zendy; Atsushi Tsuboi | Zendy; Renato Baserga | Zendy

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Effect of 5-Bromodeoxyuridine on Chromatin Transcription in Confluent Fibroblasts

Author(s) -

Bridget T. Hill,

Atsushi Tsuboi,

Renato Baserga

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.2.455

Subject(s) - rna , microbiology and biotechnology , biology , polymerase , dna , bromodeoxyuridine , nuclease protection assay , transcription (linguistics) , rnase p , chromatin , rna dependent rna polymerase , biochemistry , cell growth , gene , linguistics , philosophy

3T6 mouse fibroblasts were grown in 5-bromodeoxyuridine (BrdU) so that approximately 20% of the thymine residues in DNA were replaced by BrdU. BrdU replacement caused an alteration in the relative incorporation of labeled nucleotide precursors into RNA. The RNA synthesized by cells grown in BrdU has a lower proportion of adenine and a higher guanine complement. This was shown for (a ) nascent RNA madein vivo by confluent monolayers of cells in culture; (b ) RNA synthesizedin vitro on a chromatin template with either homologous or heterologous RNA polymerase; and (c ) RNA synthesizedin vitro on a DNA template with a highly purified RNA polymerase. The product was completely digested by RNase. The relative decrease in the incorporation of adenine into RNA was reserved when BrdU-treated cells were allowed to proliferate in BrdU-free medium.

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