In Vitro Synthesis of Ribosomal Proteins Directed by Escherichia coli DNA
Author(s) -
E. Kaltschmidt,
Lawrence Kahan,
Michio Nomura
Publication year - 1974
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.2.446
Subject(s) - ribosome , 30s , ribosomal rna , escherichia coli , ribosomal protein , 50s , dna , microbiology and biotechnology , dna polymerase , in vitro , dna polymerase i , biology , biochemistry , polymerase , chemistry , rna , gene , reverse transcriptase
In vitro synthesis of a number of E. coli 30S ribosomal proteins has been demonstrated in a cell-free system consisting of ribosomes, initiation factors, RNA polymerase, a fraction containing soluble enzymes and factors, and E. coli DNA. DNA-dependent synthesis of the following 30S proteins has been demonstrated: S4, S5, S7, S8, S9, S10, S13, S14, S16, S19, and S20.
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