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Magnetic Circular Dichroism Studies XXV. A Preliminary Investigation of Microsomal Cytochromes
Author(s) -
Peter M. Dolinger,
Michael Kielczewski,
James R. Trudell,
G Barth,
Robert E. Linder,
E. Bunnenberg,
Carl Djerassi
Publication year - 1974
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.2.399
Subject(s) - cytochrome , circular dichroism , microsome , magnetic circular dichroism , chemistry , pseudomonas putida , biochemistry , spectral line , enzyme , physics , astronomy
The application of magnetic circular dichroism as an optical probe for simultaneous identification and determination of at least two microsomal cytochromes is demonstrated. The assignments of the bands in the spectra of microsomal suspensions are made from the spectra of soluble preparations of cytochrome P-450 obtained from Pseudomonas putida and of cytochrome b(5) obtained from rat livers.

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