Open AccessTransformation by Simian Virus 40 of Spleen Cells from a Hyperimmune Rabbit: Demonstration of Production of Specific Antibody to the Immunizing Antigen
Author(s) -
A. Donny Strosberg,
Jeffrey J. Collins,
Paul H. Black,
Daniel Malamud,
Sharon M. Wilbert,
Kurt J. Bloch,
Edgar Haber
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.2.263
Subject(s) - isoelectric point , antibody , biology , isoelectric focusing , microbiology and biotechnology , antigen , transformation (genetics) , spleen , virus , agarose , virology , biochemistry , immunology , enzyme , gene
Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [125 I]S3 polysaccharide-protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti-S3 antibodies of the donor rabbit. These observations strongly suggest that a normal committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.