Open AccessDeoxysubstitution in RNA by RNA Polymerase In Vitro: A New Approach to Nucleotide Sequence Determinations
Author(s) -
Gary V. Paddock,
Howard C. Heindell,
Winston Salser
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.12.5017
Subject(s) - phosphodiester bond , rna , polymerase , nucleotide , microbiology and biotechnology , rna polymerase , dna , nucleic acid sequence , biology , in vitro , deoxyribonuclease , biochemistry , chemistry , gene
Deoxynucleotides have been incorporated into RNA synthesizedin vitro by RNA polymerase with either double-stranded or single-stranded DNA as a template. By use of this technique to block or promote cleavage at a particular phosphodiester bond, a variety of specific cleavages may be obtained with the available ribonucleases and deoxyribonuclease I. These methods should greatly increase the ease and rapidity of nucleotide sequence determinations.