Open AccessThe Presence of I-Region-Associated Antigens on B Cells in Molecules Distinct from Immunoglobulin and H-2K and H-2D
Author(s) -
Emil R. Unanue,
Martin E. Dorf,
Chella S. David,
Baruj Benacerraf
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.12.5014
Subject(s) - antigen , surface immunoglobulin , antibody , immunofluorescence , microbiology and biotechnology , biology , immunoglobulin g , pan t antigens , incubation , chemistry , b cell , immunology , monoclonal antibody , biochemistry
The distribution of I-region-associated antigenic specificities in murine lymphoid cells was studied by immunofluorescence. The antigens were mostly represented in B cells and in macrophages. The complexes of I-anti-I-region-associated antigens were found to be distributed throughout the lymphocyte surface both at 4° and 37°. In about two-thirds of the lymphocytes the complexes redistributed into caps after incubation with a second ligand (an anti-mouse immunoglobulin). The I-region specificities were in molecules distinct from immunoglobulin or from the molecules coded by the H-2K and H-2D regions.