Open AccessReplacement of Ribosomal Protein S1 by Interference Factor iα in Ribosomal Binding of Phage MS2 RNA
Author(s) -
José Miguel Hermoso,
Włodzimierz Szer
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.12.4708
Subject(s) - 30s , rna , ribosomal protein , ribosomal rna , 5s ribosomal rna , biology , microbiology and biotechnology , rna binding protein , 5.8s ribosomal rna , ribosome , biochemistry , gene
The MS2 RNA binding capacity of 30S ribosomal subunits, which is lost when protein S1 is removed, can be restored following incubation with interference factor iα and repelleting. Polyacrylamide-agarose gel electrophoresis shows that, under these conditions, a faster moving, non-RNA binding 30S species, which contains no S1, is converted to a slower moving RNA-binding one, having the same mobility as the 30S species that contains protein S1. Factor iα binds to single-stranded RNAs in a pattern that closely resembles the RNA binding pattern of initiation factor IF-3.