Open AccessLocalization of Escherichia coli Ribosomal Proteins S4 and S14 by Electron Microscopy of Antibody-Labeled Subunits
Author(s) -
James A. Lake,
Maryanne Pendergast,
Lawrence Kahan,
Masayasu Nomura
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.12.4688
Subject(s) - ribosome , protein subunit , escherichia coli , electron microscope , ribosomal protein , eukaryotic ribosome , ribosomal rna , chemistry , microbiology and biotechnology , eukaryotic large ribosomal subunit , protein quaternary structure , biochemistry , biophysics , biology , rna , gene , physics , optics
Binding sites for antibodies specific for proteins S4 and S14 of the small subunit ofE. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence ofE. coli S4 and S14. Anti-S14 IgG was found to bind to a limited region of the ribosome surface. In contrast anti-S4 IgG was found to bind to three separated regions of the ribosome surface, suggesting S4 has an elongated conformationin situ .