Open AccessMagnetic Circular Dichroism Studies XXXV. A Comparison of Cytochromes P -450 and P -448
Author(s) -
John H. Dawson,
Peter M. Dolinger,
James R. Trudell,
G Barth,
Robert E. Linder,
E. Bunnenberg,
Carl Djerassi
Publication year - 1974
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.11.4594
Subject(s) - circular dichroism , cytochrome , chemistry , spectroscopy , magnetic circular dichroism , heme , nuclear magnetic resonance , crystallography , biochemistry , physics , enzyme , spectral line , quantum mechanics , astronomy
Cytochromes P-450 and P-448 in microsomal suspensions have been shown to be spectrally distinct by magnetic circular dichroism spectroscopy. Furthermore, this technique can be used to measure induction of these two cytochromes by phenobarbital and 3-methyl-cholanthrene. Magnetic circular dichroism spectroscopy is thus at least as useful as difference spectroscopy for the investigation of P-450 and P-448 and more informative because the presence of cytochrome b(5) and hemoglobin can be detected concurrently. We have also shown that the molar magnetic ellipticity for reduced + CO treated cytochrome P-450 of Pseudomonas grown on camphor is of similar value to that of reduced + CO treated microsomal P-450 and P-448.
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