Open AccessA Pyruvate-Valine Enzyme Complex that is Dependent upon the Metabolic State of the Mitochondria
Author(s) -
Arloa Bergquist,
E. A. Eakin,
D.K. Murali,
Robert P. Wagner
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.11.4352
Subject(s) - neurospora crassa , digitonin , biochemistry , valine , enzyme , mitochondrion , biology , biosynthesis , isoleucine , neurospora , chemistry , amino acid , leucine , gene , mutant
Data are presented suggesting that intactNeurospora crassa mitochondria contain an enzyme complex incorporating five enzymes necessary for the biosynthesis of isoleucine and valine. The functional integrity and stability of the complex has been shown to be dependent on the metabolic state of the mitochondria. The complex has been solubilized by digitonin and has an approximate molecular weight of 400,000.