Elongation of the Polyadenylate Segment of Messenger RNA in the Cytoplasm of Mammalian Cells | Zendy

J. Portillo Díez | Zendy; George Brawerman | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Elongation of the Polyadenylate Segment of Messenger RNA in the Cytoplasm of Mammalian Cells

Author(s) -

J. Portillo Díez,

George Brawerman

Publication year - 1974

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.71.10.4091

Subject(s) - polyadenylation , cytoplasm , messenger rna , rna , nucleus , polynucleotide , microbiology and biotechnology , elongation , biology , biochemistry , gene , materials science , ultimate tensile strength , metallurgy

Chinese hamster and mouse sarcoma 180 ascites cells, treated with high levels of actinomycin D, still carry out limited poly(A) synthesis. The residual activity, which consists of poly(A) chain extension in the cytoplasm as well as in the nucleus, is essentially insensitive to cordycepin. Nuclear polyadenylation proceeds linearly and involves the gradual extension of unusually long poly(A) sequences. Cytoplasmic poly(A) labeling is initially more rapid than in the nucleus, but levels off within 5-10 min. It consists of addition of seven to eight AMP residues to the poly(A) sequences in preexisting mRNA molecules. The levelling off can be accounted for by a rapid turnover of the extremity of the poly(A) chain in the mRNA. Cytoplasmic poly(A) chain extension can be detected in cells not subjected to the actinomycin treatment. The rate of this process is of the same order of magnitude as that of new poly(A) transfer from nucleus to cytoplasm. It could serve to control the length of the poly(A) sequence in mRNA.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research