Open AccessInvolvement of Two Protein Factors and ATP in In Vitro DNA Synthesis Catalyzed by DNA Polymerase III of Escherichia coli
Author(s) -
Jerard Hurwitz,
Sue Wickner
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.1.6
Subject(s) - dna polymerase , dna clamp , primase , dna polymerase ii , polymerase , primer (cosmetics) , dna polymerase i , microbiology and biotechnology , deoxyribonucleoside , biology , dna , rna polymerase , escherichia coli , biochemistry , chemistry , rna , reverse transcriptase , gene , organic chemistry
Thein vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends onE. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg+2 , spermidine or DNA-unwinding protein ofE. coli , and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA)·oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.