Open AccessInt-Constitutive Mutants of Bacteriophage Lambda
Author(s) -
Kazunori Shimada,
Allan Campbell
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.1.237
Subject(s) - prophage , lysogen , lysogenic cycle , biology , bacteriophage , mutant , genetics , escherichia coli , gene , microbiology and biotechnology
The constitutive production of small amounts oftrpB enzyme in anEscherichia coli strain carrying λcI 857 prophage within thetrpC gene has been examined in derivatives of this strain from which portions of the prophage have been deleted. Enzyme production requires a site (pI ) within the prophage close to the left prophage end. Selection for mutants of this lysogen that grow on low concentrations of indole yielded two types of mutations within the prophage: (a )v 2-type, in which all phage genes controlled by the major leftward operator are derepressed; and (b )int-c type, in which the only phage gene derepressed isint . Theint-c mutations lie in the same part of the prophage as pI . Allint-c mutants appear deficient inxis gene function, even when derepressed.