Open AccessRestoration of the Conversion of Desmosterol to Cholesterol in L-Cells after Hybridization with Human Fibroblasts
Author(s) -
Carlo M. Croce,
Irene R. Kieba,
Hilary Koprowski,
Marina Molino,
George H. Rothblat
Publication year - 1974
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.71.1.110
Subject(s) - desmosterol , sterol , cholesterol , biochemistry , chemistry , biology , microbiology and biotechnology
Hybrids between different human cells (which synthesize cholesterol) and mouse cells (whose end-product of sterol synthesis is desmosterol) were analyzed for the ability to convert desmosterol to cholesterol. Conversion of [14 C]desmosterol to cholesterol and incorporation of [14 C]acetate into the end-product sterol were studied in the parental and hybrid cells. Concordant segregation of the conversion of desmosterol to cholesterol and the human chromosome F-20 was observed.